STP1333

    Evaluating Sublethal Indicators of Stress in Asiatic Clams (Corbicula fluminea) Caged in an Urban Stream

    Published: Jan 1998


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    Abstract

    Freshwater bivalves have been used extensively to monitor chemical accumulation in field exposures, although little information is available on the use of biomarker measurements in field exposures with bivalves. DNA strand breakage, growth rate, condition index and percentage tissue water were measured in freshwater Asiatic clams (Corbicula flumined) exposed in-situ in a stream that receives urban and industrial stormwater runoff and in a non-impacted reference stream. After 4 weeks exposure, DNA strand lengths in foot tissue from Trail Creek-exposed clams were significantly shorter than DNA from reference clams. These results suggest a reduction in DNA integrity in Trail Creek-exposed clams, possibly indicating exposure to genotoxic chemicals. No significant differences were observed in the growth rates of clams. However, a significant inverse relationship was detected between condition index and % tissue water for all clams. Furthermore, site-specific differences in percentage tissue water and condition indices were observed after 2 and 10 weeks exposure. For this study DNA strand breakage, condition indices, and tissue hydration appear to be more sensitive indicators of sublethal toxicity than growth.

    Keywords:

    biomarkers, DNA strand breakage, in-situ, exposures, Corbicula fluminea, nonpoint source pollution


    Author Information:

    Black, MC
    Assistant professor and graduate student, Environmental Health Science Program, College of Agricultural and Environmental Sciences, The University of Georgia, Athens, GA

    Belin, JI
    Assistant professor and graduate student, Environmental Health Science Program, College of Agricultural and Environmental Sciences, The University of Georgia, Athens, GA


    Paper ID: STP12156S

    Committee/Subcommittee: E47.09

    DOI: 10.1520/STP12156S


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