STP1306: Effect of Low-Level Copper and Pentachlorophenol Exposure on Various Early Life Stages of Xenopus Laevis

    Fort, DJ
    Vice President and President, THE STOVER GROUP, Stillwater, OK

    Stover, EL
    Vice President and President, THE STOVER GROUP, Stillwater, OK

    Pages: 16    Published: Jan 1996


    An evaluation of the effects of low-level copper and pentachlorophenol exposure on various early life stages of the South African clawed frog, Xenopus laevis, was performed using stage-specific and long-term continuous exposures. Stage-specific exposure experiments were conducted such that separate subsets of embryos and larvae from the same clutch were exposed to two toxicants, copper and pentachlorophenol, from 0 d to 4 d (standard Frog Embryo Teratogenesis Assay — Xenopus [FETAX]), 4 d to 8 d, 8 d to 12 d, and 12 d to 16 d. Results from two separate concentration-response experiments indicated that sensitivity to either toxicant increased in each successive time period. Longer-term exposure studies conducted for 60 to 75 days indicated that copper, but not pentachlorophenol induced reduction deficiency malformations of the hind limb at concentrations as low as 0.05 mg/L. Pentachlorophenol concentrations as low as 0.5 μg/L inhibited tail resorption. However, copper did not adversely affect the process of tail resorption. These results indicated that studies evaluating longer-term developmental processes are important in ecological hazard evaluation.


    X. laevis, FETAX, copper, pentachlorophenol, limb deficits, tail resorption

    Paper ID: STP11708S

    Committee/Subcommittee: E47.09

    DOI: 10.1520/STP11708S

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