Fluorescence Induction as a Biomarker of Creosote Phototoxicity to the Aquatic Macrophyte Lemna Gibba

    Published: Jan 1996

      Format Pages Price  
    PDF Version (224K) 14 $25   ADD TO CART
    Complete Source PDF (8.1M) 14 $100   ADD TO CART


    Biomarkers of polycyclic aromatic hydrocarbon (PAH) toxicity to aquatic plants were developed using the wood preservative creosote. We tested physiological indicators of photosynthetic performance in cultures of the floating aquatic macrophyte Lemna gibba (G3). Creosote was applied at concentrations ranging from 1–300 ppm, and plants were grown under laboratory lighting that mimics the relative levels of UV radiation found in natural sunlight (simulated solar radiation; SSR). Population growth bioassays demonstrated that similar to individual PAHs, creosote exhibited UV-enhanced phototoxicity. Chlorophyll content and chlorophyll fluorescence induction parameters were also diminished by creosote, and closely corresponded to functional responses of population growth by the end of each experiment. Fluorescence induction thus is a validated biomarker assay that is closely and functionally related to population growth inhibition in aquatic plants.


    Creosote, biomarkers, photosynthesis, population growth, duckweed, Lemna gibba, phototoxicity, fluorescence induction

    Author Information:

    Gensemer, RW
    Centre for Toxicology, University of Guelph, Guelph, Ontario

    Boston University, Boston, MA

    Ren, L
    University of Waterloo, Waterloo, Ontario

    Day, KE
    Centre for Toxicology, University of Guelph, Guelph, Ontario

    Canada Centre for Inland Waters, Burlington, Ontario

    Solomon, KR
    Centre for Toxicology, University of Guelph, Guelph, Ontario

    Greenberg, BM
    University of Waterloo, Waterloo, Ontario

    Paper ID: STP11706S

    Committee/Subcommittee: E47.09

    DOI: 10.1520/STP11706S

    CrossRef ASTM International is a member of CrossRef.