STP1306: Development and Characterization of an Elisa for trans-3-Hydroxycotinine, a Biomarker for Mainstream and Sidestream Smoke Exposure

    Rees, WA
    The Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky

    The Maxwell Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky

    Kwiatkowski, S
    University of Kentucky, Lexington, Kentucky

    Stanley, SD
    Truesdail Laboratories, Inc., Tustin, California

    Granstrom, DE
    The Maxwell Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky

    Yang, JM
    Duke University Medical Center, Durham, N.C.

    Gairola, CG
    The Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky

    Tobacco and Health Research Institute, University of Kentucky, Lexington, Kentucky

    Drake, D
    University of Kentucky, Lexington, Kentucky

    Glowczyk, J
    University of Kentucky, Lexington, Kentucky

    Woods, WE
    The Maxwell Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky

    Tobin, T
    The Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky

    The Maxwell Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky

    Pages: 14    Published: Jan 1996


    Abstract

    trans-3-Hydroxycotinine is the major urinary metabolite of nicotine in man and can serve as an important biomarker of tobacco smoke exposure. A sensitive ELISA test for trans-3-hydroxycotinine was developed with an I-50 for this nicotine biomarker of between 1.0–3.0 ng/ml. This ELISA test has about 10 fold less affinity for cotinine and 1000-fold less affinity for nicotine and other nicotine metabolites. No matrix effects were detectable in human saliva and relatively small matrix effects (I-50 for trans-3-hydroxycotinine, about 25 ng/ml) in urine was observed. The assay readily detected levels of apparent trans-3-hydroxycotinine in urine samples from smoke-exposed mice and rats. This ELISA is therefore a sensitive test for the determination of trans-3-hydroxycotinine in plasma, saliva, and urine samples from humans and animals, and can be used to monitor exposure to tobacco smoke or nicotine.

    Keywords:

    Enzyme-Linked Immunosorbant Assay, nicotine, t, -3-hydroxycotinine, cotinine, metabolite


    Paper ID: STP11705S

    Committee/Subcommittee: E47.09

    DOI: 10.1520/STP11705S


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