SEDL / STP / STP1452-EB / STP11643S

Biomolecules in Tissue Engineered Medical Products (TEMPs): A Case Study of Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2)

Porter, TJ
Senior Scientist, Scientist and Lab Head, Wyeth BioPharma, Andover, MA

Rathore, S
Senior Scientist, Scientist and Lab Head, Wyeth BioPharma, Andover, MA

Rouse, J
Senior Scientist, Scientist and Lab Head, Wyeth BioPharma, Andover, MA

Denton, M
Principal Scientist, Wyeth BioPharma, Andover, MA

Pages: 22    Published: Jan 2004

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Abstract

rhBMP-2 is an osteoinductive protein that, when administered locally, results in the induction of new bone tissue at the site of implantation. The osteoinductive properties of rhBMP-2 first were demonstrated by implantation at ectopic (nonbony) sites in rodents. BMPs are the only protein factors known to induce new, ectopic bone. rhBMP-2/Absorbable Collagen Sponge (ACS) is a TEMP that combines rhBMP-2 with a matrix for implantation. rhBMP-2/ACS is used in long bone fracture repair and spinal fusion. The development of rhBMP-2 protein as the active ingredient in the rhBMP-2/ACS product is summarized. Four aspects of rhBMP-2 development are covered: protein structural characterization, functional characterization, purity analysis and design of comparability studies to support changes in the manufacturing process. Topics discussed include rhBMP-2 as a “biologic” and as a constituent of a combination TEMP. The lessons learned during the development of rhBMP-2/ACS may allow general insight into the development of future standards for TEMPs that contain biologically active proteins.