STP1452

    Endpoint Verification of Bone Demineralization for Tissue Engineering Applications

    Published: Jan 2004


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    Abstract

    Demineralized bone matrix is used in tissue engineering applications due to its ability to promote new bone formation. Numerous processing variables, such as the degree of demineralization, can affect the matrix's bone-forming ability. Several methods exist for determining the extent of demineralization, but each method has disadvantages. This study investigated the potential use of a stereomicroscope in determining the degree of demineralization by comparing the novel technique to two conventional methods. Bone fragments were processed and demineralized using a standard protocol, and their degree of demineralization was characterized over time by the three methods. The results indicate that a stereomicroscopy method is as effective as two established methods in verifying the degree of demineralization.

    Keywords:

    tissue engineering, demineralized bone matrix, stereomicroscope, endpoint determination


    Author Information:

    Thomas, CB
    Graduate research assistant, histotechnologist, and assistant professor, Clemson University, Clemson, SC

    Jenkins, L
    Graduate research assistant, histotechnologist, and assistant professor, Clemson University, Clemson, SC

    Kellam, JF
    Vice Chairman, Carolinas Medical Center, Charlotte, NC

    Burg, KJL
    Graduate research assistant, histotechnologist, and assistant professor, Clemson University, Clemson, SC


    Paper ID: STP11639S

    Committee/Subcommittee: F04.43

    DOI: 10.1520/STP11639S


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