SEDL / STP / STP1452-EB / STP11639S

Endpoint Verification of Bone Demineralization for Tissue Engineering Applications

Thomas, CB
Graduate research assistant, histotechnologist, and assistant professor, Clemson University, Clemson, SC

Jenkins, L
Graduate research assistant, histotechnologist, and assistant professor, Clemson University, Clemson, SC

Kellam, JF
Vice Chairman, Carolinas Medical Center, Charlotte, NC

Burg, KJL
Graduate research assistant, histotechnologist, and assistant professor, Clemson University, Clemson, SC

Pages: 10    Published: Jan 2004

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Abstract

Demineralized bone matrix is used in tissue engineering applications due to its ability to promote new bone formation. Numerous processing variables, such as the degree of demineralization, can affect the matrix's bone-forming ability. Several methods exist for determining the extent of demineralization, but each method has disadvantages. This study investigated the potential use of a stereomicroscope in determining the degree of demineralization by comparing the novel technique to two conventional methods. Bone fragments were processed and demineralized using a standard protocol, and their degree of demineralization was characterized over time by the three methods. The results indicate that a stereomicroscopy method is as effective as two established methods in verifying the degree of demineralization.