STP1452

    Cartilage Mechanical Properties after Injury

    Published: Jan 2004


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    Abstract

    Cartilage injury often results in matrix degradation and in secondary osteoarthritis. This study was designed to correlate cell death and matrix degradation with biomechanical properties of cartilage. Full-thickness mature bovine femoral articular cartilage was harvested as 5 mm diameter cylindrical disk explants. Explants were divided into three groups: control, injury, and IL-1. The injury group was subjected to mechanical compression of 40% strain for five minutes. The IL-1 group was cultured in media containing 10 ng/mL of IL-1 beta. The control group was not injured or exposed to IL-1 beta. Chondrocyte viability, glycosaminoglycan release in media, and equilibrium creep were measured ten days after injury. A reduction in cell viability was seen after injury. A significant increase in glycosaminoglycan release and in equilibrium creep was detected in injured explants and in explants exposed to IL-1 beta. A correlation was also found between the equilibrium creep and glycosaminoglycan content after injury and IL-1 beta stimulation.

    Keywords:

    Chondrocyte, cartilage, injury, viability, biomechanics, biomechanical properties, elastic modulus, glycosaminoglycan, trauma, cartilage repair, cartilage degeneration, cartilage lesion


    Author Information:

    D'Lima, DD
    Director, Research Associates, and Senior Bioengineer, Orthopaedic Research Laboratories, Scripps Clinic Center for Orthopaedic Research and Education, La Jolla, CA

    Professor, Scripps Research Institute, La Jolla, CA

    Steklov, N
    Director, Research Associates, and Senior Bioengineer, Orthopaedic Research Laboratories, Scripps Clinic Center for Orthopaedic Research and Education, La Jolla, CA

    Bergula, A
    Director, Research Associates, and Senior Bioengineer, Orthopaedic Research Laboratories, Scripps Clinic Center for Orthopaedic Research and Education, La Jolla, CA

    Chen, PC
    Director, Research Associates, and Senior Bioengineer, Orthopaedic Research Laboratories, Scripps Clinic Center for Orthopaedic Research and Education, La Jolla, CA

    Colwell, CW
    Director, Scripps Clinic Center for Orthopaedic Research and Education, La Jolla, CA

    Lotz, M
    Professor, Scripps Research Institute, La Jolla, CA


    Paper ID: STP11636S

    Committee/Subcommittee: F04.15

    DOI: 10.1520/STP11636S


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