STP1452

    Towards In-Situ Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques

    Published: Jan 2004


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    Abstract

    Inability to obtain cellular-level information about progress of cell growth in tissue-engineering (TE) scaffolds is a pervasive problem. We demonstrate that a confocal microscope with two collinear contrast mechanisms, optical coherence, and fluorescence, can be used to perform non-destructive imaging of a polymer TE scaffold containing osteoblasts. We show that the combination of the techniques shows promise for in situ measurements of cell growth in a bioreactor, even for highly opaque TE scaffolds.

    Keywords:

    coherence microscopy, fluorescence microscopy, morphology, OCT, tissue engineering scaffold


    Author Information:

    Cicerone, MT
    Research Scientists, National Institute of Standards and Technology, Gaithersburg, MD

    Dunkers, JP
    Research Scientists, National Institute of Standards and Technology, Gaithersburg, MD

    Washburn, NR
    Research Scientists, National Institute of Standards and Technology, Gaithersburg, MD


    Paper ID: STP11635S

    Committee/Subcommittee: F04.04

    DOI: 10.1520/STP11635S


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