SEDL / STP / STP1452-EB / STP11630S

Development and Validation of a Detection Method for a Broad Range of Human Papillomavirus Types

Galbraith, DN
Director of Technical Services and Regulatory Affairs, Project Manager, Laboratory Manager, Study Director, Study Director and Director of Development, Q-One Biotech Ltd., West of Scotland Science Park, Glasgow

Collins, T
Director of Technical Services and Regulatory Affairs, Project Manager, Laboratory Manager, Study Director, Study Director and Director of Development, Q-One Biotech Ltd., West of Scotland Science Park, Glasgow

Black, J
Director of Technical Services and Regulatory Affairs, Project Manager, Laboratory Manager, Study Director, Study Director and Director of Development, Q-One Biotech Ltd., West of Scotland Science Park, Glasgow

McManus, B
Director of Technical Services and Regulatory Affairs, Project Manager, Laboratory Manager, Study Director, Study Director and Director of Development, Q-One Biotech Ltd., West of Scotland Science Park, Glasgow

McMutrie, D
Director of Technical Services and Regulatory Affairs, Project Manager, Laboratory Manager, Study Director, Study Director and Director of Development, Q-One Biotech Ltd., West of Scotland Science Park, Glasgow

Lovatt, A
Director of Technical Services and Regulatory Affairs, Project Manager, Laboratory Manager, Study Director, Study Director and Director of Development, Q-One Biotech Ltd., West of Scotland Science Park, Glasgow

Pages: 8    Published: Jan 2004

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Abstract

Human papillomaviruses (HPVs) are viruses that are almost unique in their tropism for human skin cells; they also have the potential to cause severe pathology. These viruses present a significant risk to contaminate tissue engineered skin replacement products that use human-derived skin cells as part of their manufacture. Eight pairs of primers and appropriate DNA oligonucleotide probes were designed from a relatively conserved region of the LI open reading frame of HPV. TaqMan® PCR using these primer/probe sets was found to be capable of detecting HPV-DNA in a background of cellular material likely to be present in skin replacement products. The assay is more sensitive than any currently described in the scientific literature and can be completed in a single working day making it suitable for testing the short shelf life that many of these products have.