STP1007

    Use of Fractionation and Chemical Analysis Schemes for Plant Effluent Toxicity Evaluations

    Published: Jan 1988


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    Abstract

    A new field of analysis has arisen for the evaluation of wastewater samples in response to forthcoming regulations. Instead of using current methodology to determine if target compounds are present in environmental water samples (for example, priority pollutant analysis), Monsanto's Environmental Sciences Center (ESC) has developed a scheme to identify compounds that are either toxic to aquatic organisms or major sources of total organic carbon in wastewaters. This procedure may be necessary with the upcoming toxicity reduction evaluation (TRE) program sponsored by the United States Environmental Protection Agency (USEPA). The ESC scheme uses simple column chromatography techniques to initially fractionate the wastewater into separate inorganic and organic constituents. Each fraction is evaluated for toxicity to a common test organism, Daphnia magna. The fraction containing the toxicity is then analyzed to identify the sources of toxicity. Several case studies are presented to show how the ESC fractionation and chemical analysis scheme has been used to isolate and identify the factors responsible for wastewater toxicity.

    Keywords:

    chemical fractionation, toxicity identification, effluent toxicity, toxicity reduction evaluation, aquatic toxicology


    Author Information:

    Doi, J
    Senior reserch chemist and senior environmental specialist, Monsanta Co., Evironmental Sciences Centar, St. Louis, MO

    Grothe, DR
    Senior reserch chemist and senior environmental specialist, Monsanta Co., Evironmental Sciences Centar, St. Louis, MO


    Paper ID: STP10285S

    Committee/Subcommittee: E47.01

    DOI: 10.1520/STP10285S


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