STP1005

    Microbiological Contamination: Evaluation of Some Sampling and Laboratory Analytical Methods

    Published: Jan 1988


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    Abstract

    The limitations and problems of representative sampling in naval fuel tanks have been investigated. Problems have been associated with access, with possibly atypical habitats (for example, entrained sounding tube or drain line samples), and with types of samplers available. In laboratory bottle scale trials for biocide efficacy, microbial interface growth has been quantified by biochemical determination of cellular protein following alkali extraction. This method is applicable to frozen stored samples. The significance of mixed microbial populations, in relation to coalescer impairment and to biocide treatment of the fuel, is also discussed.

    Keywords:

    microbiological contamination, fungi, distillate fuels, Cladosporium resinae, fuel tank, sampling, protein analysis, biocides, biomass


    Author Information:

    Hebda, AJ
    Senior staff biologist and microscopist, Seatech Investigation Services, Ltd., Halifax, Nova Scotia

    Morchat, RM
    Defence Scientist, Defence Research Establishment Atlantic, Halifax, Nova Scotia

    Brown, RG
    Professor, Dalhousie University, Halifax, Nova Scotia

    Jones, GM
    Senior staff biologist and microscopist, Seatech Investigation Services, Ltd., Halifax, Nova Scotia


    Paper ID: STP10039S

    Committee/Subcommittee: D02.E0

    DOI: 10.1520/STP10039S


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