ISSN: 1945-7553
CODEN: JTEVAB
Page Count: 4

Some Factors Influencing the Enumeration of Metabolizing Aquatic Bacteria

Rao, SS
Research scientist, research technologist, and research scientist, Microbiology Laboratories Section, National Water Research Institute, Canada Centre for Inland Waters, Burlington, Ontario

Jurkovic, AA
Research scientist, research technologist, and research scientist, Microbiology Laboratories Section, National Water Research Institute, Canada Centre for Inland Waters, Burlington, Ontario

Dutka, BJ
Research scientist, research technologist, and research scientist, Microbiology Laboratories Section, National Water Research Institute, Canada Centre for Inland Waters, Burlington, Ontario

Abstract

Effects of incubation time, temperature, and organic substrate on the enumeration of total and metabolizing aquatic bacteria were evaluated with a reduction technique using 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride and formazan. Data indicated that there were no apparent differences in metabolizing bacterial numbers when water samples were incubated for up to 24 h at 4 or 20°C with or without the addition of glucose. However, increasing numbers of detectable metabolizing bacteria were noted when the water samples were incubated at 37°C for 4 h with or without added glucose. Implications of the observations as they relate to point source nutrient discharges on the nearshore-offshore bacterial populations are discussed.

Keywords:
aquatic microbiology, bacteria, enumeration

Paper ID: JTE11424J
DOI: 10.1520/JTE11424J
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