Volume 12, Issue 1 (January 1984)
Some Factors Influencing the Enumeration of Metabolizing Aquatic Bacteria
Effects of incubation time, temperature, and organic substrate on the enumeration of total and metabolizing aquatic bacteria were evaluated with a reduction technique using 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride and formazan. Data indicated that there were no apparent differences in metabolizing bacterial numbers when water samples were incubated for up to 24 h at 4 or 20°C with or without the addition of glucose. However, increasing numbers of detectable metabolizing bacteria were noted when the water samples were incubated at 37°C for 4 h with or without added glucose. Implications of the observations as they relate to point source nutrient discharges on the nearshore-offshore bacterial populations are discussed.