Volume 12, Issue 6 (November 1984)

    Evaluation of the Ultrasonic Method for Solubilizing Dapnia magna Before Liquid Scintillation Counting

    CODEN: JTEOAD

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    Abstract

    Adult Daphnia magna were exposed to 14C-labeled phenol and tissues analyzed for 14C uptake by three methods: (1) tissue solubilizer, (2) tissue solubilizer plus sonication, and (3) sonication alone. Analysis by liquid scintillation counting revealed that measurements of total activity among treatments were not significantly different (α ≤ 0.10) at two count levels. Sonicated samples showed less variation than tissue samples that were solubilized.


    Author Information:

    Dauble, DD
    Research scientist, research specialist, and research scientist, Pacific Northwest Laboratory, Richland, WA

    Hanf, RW
    Research scientist, research specialist, and research scientist, Pacific Northwest Laboratory, Richland, WA

    Carlile, DW
    Research scientist, research specialist, and research scientist, Pacific Northwest Laboratory, Richland, WA


    Stock #: JTE10744J

    ISSN: 0090-3973

    DOI: 10.1520/JTE10744J

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    Author
    Title Evaluation of the Ultrasonic Method for Solubilizing Dapnia magna Before Liquid Scintillation Counting
    Symposium , 0000-00-00
    Committee E47