ISSN: 1945-7553
CODEN: JTEVAB
Page Count: 2

Evaluation of the Ultrasonic Method for Solubilizing Dapnia magna Before Liquid Scintillation Counting

Dauble, DD
Research scientist, research specialist, and research scientist, Pacific Northwest Laboratory, Richland, WA

Hanf, RW
Research scientist, research specialist, and research scientist, Pacific Northwest Laboratory, Richland, WA

Carlile, DW
Research scientist, research specialist, and research scientist, Pacific Northwest Laboratory, Richland, WA

Abstract

Adult Daphnia magna were exposed to ¹⁴C-labeled phenol and tissues analyzed for ¹⁴C uptake by three methods: (1) tissue solubilizer, (2) tissue solubilizer plus sonication, and (3) sonication alone. Analysis by liquid scintillation counting revealed that measurements of total activity among treatments were not significantly different (α ≤ 0.10) at two count levels. Sonicated samples showed less variation than tissue samples that were solubilized.

Keywords:
aquatic animals, aquatic biology, tissues (biology), biouptake, sonication, tissue solubilizer, liquid scintillation, Daphnia magna

Paper ID: JTE10744J
DOI: 10.1520/JTE10744J
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