ISSN: 1945-7553
CODEN: JTEVAB
Page Count: 4

Development of a Multiple Inoculator System for the Identification of Aerobic Heterotrophic Bacteria

Seyfried, PL
Professor and undergraduate studentmember of ASTM, University of Toronto, Toronto, Ontario

Pickersgill, AM
Professor and undergraduate studentmember of ASTM, University of Toronto, Toronto, Ontario

Vlassoff, LT
Manager of the Microbiology Section and manager of the Aquatic and Terrestial Ecosystems Section, Laboratory Services and Applied Research Branch, Ministry of the Environment, Rexdale, Ontario

Pagel, JE
Manager of the Microbiology Section and manager of the Aquatic and Terrestial Ecosystems Section, Laboratory Services and Applied Research Branch, Ministry of the Environment, Rexdale, Ontario

Abstract

A semi-automated multiple inoculator system was modified and evaluated for use with 25-compartment petri dishes. Twenty-one species of environmental and culture collection bacteria were tested for the following characteristics: nitrate reduction, indole production, methyl red reaction, acid from glucose, gelatin hydrolysis, phenylalanine deaminase, citrate utilization, aesculin hydrolysis, reactions on skim milk agar, arginine dihydrolase, and urea hydrolysis. The agreement between the inoculator system and conventional methods was 90 to 100% for all tests except arginine dihydrolase and urea hydrolysis. The multiple inoculator described was judged a simple and versatile device for the rapid identification of large numbers of bacteria.

Keywords:
bacteria, inoculation, aerobic bacteria, multiple inoculator, Repli dish, heterotrophic bacteria

Paper ID: JTE10724J
DOI: 10.1520/JTE10724J
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