Effect of Microbial Contamination of Water-Based Metalworking Fluids on the Aerosolization of Particles and Microbial Fragments

    Volume 2, Issue 8 (September 2005)

    ISSN: 1546-962X

    CODEN: JAIOAD

    Published Online: 22 June 2005

    Page Count: 7


    Reponen, T
    Associate Professor, Graduate Assistant, and Professor, University of Cincinnati, Cincinnati, Ohio

    Wang, HX
    Associate Professor, Graduate Assistant, and Professor, University of Cincinnati, Cincinnati, Ohio

    Grinshpun, SA
    Associate Professor, Graduate Assistant, and Professor, University of Cincinnati, Cincinnati, Ohio

    (Received 5 October 2004; accepted 15 March 2005)

    Abstract

    Aerosolization of particles from metalworking fluids (MWFs) was studied using a laboratory-scale set-up that simulates grinding operations. Semi-synthetic MWF and soluble oil were contaminated with Pseudomonas fluorescens, and the aerosolized particles were measured using a photometric mass monitor, optical particle counter, and a condensation nucleus counter. Microbial contamination of both semi-synthetic MWF and soluble oil increased the mass concentration as measured by the photometric aerosol mass monitor and the fine particle number concentration as measured by the condensation nucleus counter. These effects were seen most clearly for the fine size range of particles aerosolized from contaminated semi-synthetic MWF. Endotoxin results indicate that the increase in the fine particle concentration occurs at least partially due to the increase in the microbial cell wall components (fragments).


    Paper ID: JAI12838

    DOI: 10.1520/JAI12838

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    Author
    Title Effect of Microbial Contamination of Water-Based Metalworking Fluids on the Aerosolization of Particles and Microbial Fragments
    Symposium Recovery of Mycobacteria, 2004-12-07
    Committee E34