Evaluation of Chlorella Minutissima Oil for Biodiesel Production

    Volume 9, Issue 5 (May 2012)

    ISSN: 1546-962X

    CODEN: JAIOAD

    Published Online: 1 May 2012

    Page Count: 6


    Kaul, Savita
    Council of Scientific and Industrial Research, Indian Institute of Petroleum, Dehradun,

    Jain, Rahul
    Council of Scientific and Industrial Research, Indian Institute of Petroleum, Dehradun,

    Konathala, Sivakumar
    Council of Scientific and Industrial Research, Indian Institute of Petroleum, Dehradun,

    Bangwal, Dinesh
    Council of Scientific and Industrial Research, Indian Institute of Petroleum, Dehradun,

    Atray, Neeraj
    Council of Scientific and Industrial Research, Indian Institute of Petroleum, Dehradun,

    Kumar, Basant
    Council of Scientific and Industrial Research, Indian Institute of Petroleum, Dehradun,

    (Received 14 September 2011; accepted 27 March 2012)

    Abstract

    Present work is an attempt to focus on screening of microalgae chlorella minutissima, extraction of oil, and conversion of oil into biodiesel. The experimental results are supported by scanning electron microscopy/energy dispersive x-ray spectroscopy (SEM/EDX), high-performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC-MS) analytical techniques. The SEM/EDX results indicate microalgae contain several elements that are required for lipid metabolism, e.g., calcium, magnesium, iron, potassium, and nitrogen. HPLC analysis of hexane extracted oil shows the presence of 74.27 % of free fatty acids, 10.65 % of fatty acid esters, and 13.74 % triglycerides, besides unidentified component 1.34 %. These results are in close agreement with the high acid value of the oil. The presence of fatty acid esters in the oil were also confirmed by GC-MS analysis. Under optimized conditions, of acid catalyzed transesterification >97 % conversion of microalgae oil into FAME is observed. The studies indicate that lower reaction time is favorable for conversion to FAMEs. However, for the purpose of biodiesel feedstock, any increase in the lipid content of the algae will improve the efficiency of the process.


    Paper ID: JAI104322

    DOI: 10.1520/JAI104322

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    Author
    Title Evaluation of Chlorella Minutissima Oil for Biodiesel Production
    Symposium , 0000-00-00
    Committee D02