Luminescent Bacteria as an Indicator Species for Lubricant Formulation Ecotoxicity

    Volume 9, Issue 1 (January 2012)

    ISSN: 1546-962X

    CODEN: JAIOAD

    Published Online: 1 October 2011

    Page Count: 5


    Sander, John
    Lubrications Engineers, Inc.,

    Smith, Terry
    Lubrication Engineers, Inc.,

    Bilberry, Patrick
    Lubrication Engineers, Inc.,

    (Received 22 March 2011; accepted 16 August 2011)

    Abstract

    For a number of years, luminescent bacteria have been used as an indicator species for acute toxicity of substances in aquatic marine environments. Various other higher organisms have been used for this exact same purpose. Many of the higher organism test evaluations have been used successfully, but they have a few limitations, such as a low number of organisms exposed to toxicant during a test, less than desirable precision and the need for highly trained individuals to run the tests. One luminescent bacterial bioassay includes many organisms, can be run rapidly and requires training in sample dilution techniques. ASTM Subcommittee D02.12 has been working to develop an ecotoxicity method to evaluate the effects of lubricant formulations on luminescent bacteria. The theory describing this method, as well as the D02.12 method development activities, will be discussed.


    Paper ID: JAI103673

    DOI: 10.1520/JAI103673

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    Author
    Title Luminescent Bacteria as an Indicator Species for Lubricant Formulation Ecotoxicity
    Symposium , 0000-00-00
    Committee D02