ISSN: 1546-962X
Published Online: 20 May 2008
Page Count: 10

Performance of An Enzyme Electrode Designed for a Sulfide Monitoring Biosensor

Sooknah, Reeta
CANMET Materials Technology Laboratory, Natural Resources Canada,Ontario,

Papavinasam, Sankara
CANMET Materials Technology Laboratory, Natural Resources Canada,Ontario,

Attard, Michael
CANMET Materials Technology Laboratory, Natural Resources Canada,Ontario,

Revie, R. Winston
CANMET Materials Technology Laboratory, Natural Resources Canada,Ontario,

Gould, W. Douglas
CANMET Mining and Mineral Science Laboratories, Natural Resources Canada,Ontario,

Dinardo, Orlando
CANMET Mining and Mineral Science Laboratories, Natural Resources Canada,Ontario,

(Received 18 May 2007; accepted 22 April 2008)

Abstract

The construction and evaluation of an enzyme electrode designed for a sulfide monitoring biosensor, are reported in this work. The enzyme, which is immobilized in a carbon (graphite) paste with 1,1′ dimethylferrocene, catalyzes the oxidation of sulfide to sulfur.

Amperometric measurements were carried out at room temperature, and a fixed potential of 0.3 V with reference to a saturated calomel electrode, using Tris-HCl buffer (pH 7.5) as the electrolyte, and sodium sulfide solution as the substrate. Under deaerated conditions, the biosensor responded linearly to the tested sulfide concentration range of 0–4mM, giving an average apparent Michaelis constant of 3mM, and maximum current responses between 0.7 and 3.6 μA. The sensitivity of the electrodes tested ranged from 0.2–0.3 μA mM⁻¹.

Keywords:
Sulfide monitoring, electrochemical biosensor, enzyme electrode, sulfide oxidase

Paper ID: JAI101240
DOI: 10.1520/JAI101240
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