Volume 5, Issue 6 (June 2008)

    Performance of An Enzyme Electrode Designed for a Sulfide Monitoring Biosensor

    (Received 18 May 2007; accepted 22 April 2008)

    Published Online: 2008

    CODEN: JAIOAD

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    Abstract

    The construction and evaluation of an enzyme electrode designed for a sulfide monitoring biosensor, are reported in this work. The enzyme, which is immobilized in a carbon (graphite) paste with 1,1′ dimethylferrocene, catalyzes the oxidation of sulfide to sulfur.

    Amperometric measurements were carried out at room temperature, and a fixed potential of 0.3 V with reference to a saturated calomel electrode, using Tris-HCl buffer (pH 7.5) as the electrolyte, and sodium sulfide solution as the substrate. Under deaerated conditions, the biosensor responded linearly to the tested sulfide concentration range of 0–4mM, giving an average apparent Michaelis constant of 3mM, and maximum current responses between 0.7 and 3.6 μA. The sensitivity of the electrodes tested ranged from 0.2–0.3 μA mM−1.


    Author Information:

    Sooknah, Reeta
    CANMET Materials Technology Laboratory, Natural Resources Canada, Ottawa, Ontario

    Papavinasam, Sankara
    CANMET Materials Technology Laboratory, Natural Resources Canada, Ottawa, Ontario

    Attard, Michael
    CANMET Materials Technology Laboratory, Natural Resources Canada, Ottawa, Ontario

    Revie, R. Winston
    CANMET Materials Technology Laboratory, Natural Resources Canada, Ottawa, Ontario

    Gould, W. Douglas
    CANMET Mining and Mineral Science Laboratories, Natural Resources Canada, Ottawa, Ontario

    Dinardo, Orlando
    CANMET Mining and Mineral Science Laboratories, Natural Resources Canada, Ottawa, Ontario


    Stock #: JAI101240

    ISSN: 1546-962X

    DOI: 10.1520/JAI101240

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    Author
    Title Performance of An Enzyme Electrode Designed for a Sulfide Monitoring Biosensor
    Symposium Advances in Electrochemical Techniques for Corrosion Monitoring and Measurement, 2007-05-23
    Committee G01