Volume 50, Issue 6 (November 2005)

    Haplotype Distribution of the Multi-copy Y-STR Loci DYS385, DYS459 and DYS464 in Chinese Han Population

    (Received 12 April 2005; accepted 22 June 2005)

    Published Online: September

    CODEN: JFSOAD

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    Abstract

    EDTA-blood samples were collected from 176 healthy unrelated males of Han population living in Wuhan, China. DNA was extracted using Cheles-100 method (1). PCR was performed in a total volume of 10 µL containing 2–5 ng genomic DNA, 0.2 µM each primer, 10 mM Tris-HCl buffer (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, 200 µM each dNTP, and 1 U AmpliTaq Gold® DNA polymerase (Applied Biosystems, Foster City, CA). DYS459 and DYS464 were co-amplified in a duplex reaction and DYS385 was amplified in a singleples reaction. Primer sequences: DYS385: 5'-FAM-agcatgggtgacagagcta-3', 5'-gccaattacatagtcctcctttc-3'; DYS459: 5'FAM-caggtgaactggggtaaataat-3', 5'-gttgagcaacagagcaagactta-3'; DYS464: 5'-FAM-ctttgggctatgcctcagttt-3', 5'-gccatacctgggtaacagagagac-3'. PCR cycling conditions: 95°C for 11 min soak, 30 cycles of 40 s at 94°C, 40 s at 60°C, 50 s at 72°C followed by a 6 min extension period at 72°C. All loci were amplified in a GeneAmp PCR System 9700 (PE Applied Biosystems).


    Author Information:

    Zhu, C
    Wuhan Institute of Criminal Science and Technology, Wuhan,

    Huang, D
    Huazhong University of Science and Technology, Wuhan,

    Jiang, H
    Huazhong University of Science and Technology, Wuhan,

    Yin, H
    Huazhong University of Science and Technology, Wuhan,

    Yang, Q
    Huazhong University of Science and Technology, Wuhan,

    Zhai, X
    Huazhong University of Science and Technology, Wuhan,


    Stock #: JFS2005261

    ISSN: 0022-1198

    DOI: 10.1520/JFS2005261

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    Author
    Title Haplotype Distribution of the Multi-copy Y-STR Loci DYS385, DYS459 and DYS464 in Chinese Han Population
    Symposium , 0000-00-00
    Committee E30