Volume 50, Issue 6 (November 2005)
Population Data of 15 STR in Chinese Han Population From North of Guangdong
The samples were obtained from 102 unrelated, healthy individuals of Chinese Han population living in north of Guangdong province of China. Genomic DNA was extracted using the Chelex100 protocol as described by Walsh et al. (1). Fifteen STR loci and Amelogenin locus were co-amplified by using the AmpFLSTR Identifiler kit following the amplification conditions recommended by the manufacturer. Detection and genotyping of all PCR products were accomplished using ABI3100 DNA Genetic Analyzer (Applied Biosystem). Allele designation was done using GeneScan 3.7 and Genotyper 3.7. Evaluation of Hardy—Weinberg equilibrium expectations was carried out using the exact test and further statistical parameters of forensic interest were determined by using Arlequin version 1.1 (2).