Volume 50, Issue 6 (November 2005)
Allele Frequency Distribution of Five X-Chromosomal STR Loci in an Antioquian Population Sample (Colombia)
Blood samples from unrelated Caucasian individuals from Antioquia (Colombia) were collected. DNA was extracted from 200 µL of peripherical blood by the salting-out procedure (1). The primer sequences of loci and cycling conditions were as recommended in previous studies (2–4) o from Genome Database (http://www.gdb.org). The PCR products were analyzed using denaturing 4% acrylamidebis-acrilamide gel electrophoresis and detected by silver staining. Alleles were identified based on the number of repeats and their attribution was made by comparison with an in-house constructed allelic ladder and following the published nomenclature (2–4) and ISFG guidelines for STR analysis (5).