ISSN: 0022-1198
CODEN: JFSCA
Published Online: 7 September 2005
Page Count: 2

Allele Frequency Distribution of Five X-Chromosomal STR Loci in an Antioquian Population Sample (Colombia)
Espinal, C
GENES Ltda.,

Aguirre, D
GENES Ltda.,

Builes, JJ

Moreno, MA

Bravo, M
GENES Ltda.,

Jaramillo, P
Universidad de Antioquia,

(Received 12 April 2005; accepted 22 June 2005)

Abstract

Blood samples from unrelated Caucasian individuals from Antioquia (Colombia) were collected. DNA was extracted from 200 µL of peripherical blood by the salting-out procedure (1). The primer sequences of loci and cycling conditions were as recommended in previous studies (2–4) o from Genome Database (http://www.gdb.org). The PCR products were analyzed using denaturing 4% acrylamidebis-acrilamide gel electrophoresis and detected by silver staining. Alleles were identified based on the number of repeats and their attribution was made by comparison with an in-house constructed allelic ladder and following the published nomenclature (2–4) and ISFG guidelines for STR analysis (5).

Keywords:
forensic science, DNA typing, population genetics, X-Chromosome, STR, DXS6798, DXS6807, DXS7423, DXS6800, DXS8377, Antioquia, Colombia

Paper ID: JFS2005248
DOI: 10.1520/JFS2005248
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