Allele Frequency Distribution of Five X-Chromosomal STR Loci in an Antioquian Population Sample (Colombia)

    Volume 50, Issue 6 (November 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 7 September 2005

    Page Count: 2


    Espinal, C
    GENES Ltda., Medellín,

    Aguirre, D
    GENES Ltda., Medellín,

    Builes, JJ

    Moreno, MA

    Bravo, M
    GENES Ltda., Medellín,

    Jaramillo, P
    Universidad de Antioquia, Medellín,

    (Received 12 April 2005; accepted 22 June 2005)

    Abstract

    Blood samples from unrelated Caucasian individuals from Antioquia (Colombia) were collected. DNA was extracted from 200 µL of peripherical blood by the salting-out procedure (1). The primer sequences of loci and cycling conditions were as recommended in previous studies (2–4) o from Genome Database (http://www.gdb.org). The PCR products were analyzed using denaturing 4% acrylamidebis-acrilamide gel electrophoresis and detected by silver staining. Alleles were identified based on the number of repeats and their attribution was made by comparison with an in-house constructed allelic ladder and following the published nomenclature (2–4) and ISFG guidelines for STR analysis (5).


    Paper ID: JFS2005248

    DOI: 10.1520/JFS2005248

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    Title Allele Frequency Distribution of Five X-Chromosomal STR Loci in an Antioquian Population Sample (Colombia)
    Symposium , 0000-00-00
    Committee E30