Unlinked Tetrameric Microsatellites on the X Chromosome: Frequency Data in Males from Cantabria (Northern Spain)

    Volume 50, Issue 6 (November 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 14 September 2005

    Page Count: 2


    Zarrabeitia, AL
    University of Cantabria,

    Alonso, A
    University of Cantabria,

    Riancho, JA
    University of Cantabria,

    Blanco, L
    University of Cantabria,

    Zarrabeitia, M
    University of Cantabria,

    (Received 12 April 2005; accepted 22 June 2005)

    Abstract

    We studied unrelated male donors (n = 141) living in Cantabria, a region in northern Spain. DNA was extracted from peripheral blood by using the Qiagen blood kit (Qiagen, Hilden, Germany). Loci of interests in the X-chromosome (DXS9895, GATA172D05 and DXS9898) were amplified in a single PCR with primers of published sequences (1-3). PCR conditions consisted of an initial denaturation step at 94° for 10 min, followed by eight cycles with denaturation at 94° for 1 min, annealing at decreasing temperature between 62° and 59° (1° decrease every two cycles) and extension at 72° for 1 min. Then 24 cycles at 94° 1 min, 58° 1 min, 72° 1 min; followed by a final extension at 72° for 30 min. PCR products were analyzed by capillary electrophoresis (ABI310, Applied Biosystems), with sequenced controls. Allele designation was as reported previously (1,2).


    Paper ID: JFS2005238

    DOI: 10.1520/JFS2005238

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    Author
    Title Unlinked Tetrameric Microsatellites on the X Chromosome: Frequency Data in Males from Cantabria (Northern Spain)
    Symposium , 0000-00-00
    Committee E30