ISSN: 0022-1198
CODEN: JFSCA
Published Online: 7 September 2005
Page Count: 3

Autosomal STR Frequencies in Afghanistan Population
Berti, A
Carabinieri Scientific Investigation Department of Rome-DNA Unit,

Di Carlo, A
Carabinieri Scientific Investigation Department of Rome-Fingerprint Unit,

Virgili, A
Carabinieri Scientific Investigation Department of Rome-DNA Unit,

Oddo, CM
Carabinieri Headquarter-Healt Department,

Franchi, C
Carabinieri Scientific Investigation Department of Rome-DNA Unit,

Rapone, C
Carabinieri Scientific Investigation Department of Rome-DNA Unit,

Barni, F
Carabinieri Scientific Investigation Department of Rome-DNA Unit,

Iacovacci, G
Carabinieri Scientific Investigation Department of Rome-DNA Unit,

Lago, G
Carabinieri Scientific Investigation Department of Rome-DNA Unit,

(Received 12 April 2005; accepted 22 June 2005)

Abstract

Buccal cells were collected by buccal brushes (Sterile Omni Swab, Whatman International Ltd.) from healthy, random Afghanian individuals. DNA was extracted by Chelex method (1). DNA samples were quantified using AmpFℓSTR® Quantifiler (Ap plied Biosystems, Foster City, CA) by Real time 7000 (Applied Biosystems, Foster City, CA), one nanogram of the samples obtained from the previous phase was amplified by GeneAmp® PCR System 9600 (Applied Biosystems, Foster City, CA). Simultaneous amplifications of 16 STRs loci (multiplexed PCR) were performed by using AmpFℓSTR® Identifiler™ (Applied Biosystems, Foster City, CA) according to the user manual recommendations (2).

Keywords:
forensic science, DNA typing, population genetics, STR frequencies, Afghanistan

Paper ID: JFS2005237
DOI: 10.1520/JFS2005237
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