Journal Published Online: 07 September 2005
Volume 50, Issue 6

Autosomal STR Frequencies in Afghanistan Population

CODEN: JFSCAS

Abstract

Buccal cells were collected by buccal brushes (Sterile Omni Swab, Whatman International Ltd.) from healthy, random Afghanian individuals. DNA was extracted by Chelex method (1). DNA samples were quantified using AmpFℓSTR® Quantifiler (Ap plied Biosystems, Foster City, CA) by Real time 7000 (Applied Biosystems, Foster City, CA), one nanogram of the samples obtained from the previous phase was amplified by GeneAmp® PCR System 9600 (Applied Biosystems, Foster City, CA). Simultaneous amplifications of 16 STRs loci (multiplexed PCR) were performed by using AmpFℓSTR® Identifiler™ (Applied Biosystems, Foster City, CA) according to the user manual recommendations (2).

Author Information

Berti, A
Carabinieri Scientific Investigation Department of Rome-DNA Unit, Rome, Italy
Barni, F
Carabinieri Scientific Investigation Department of Rome-DNA Unit, Rome, Italy
Virgili, A
Carabinieri Scientific Investigation Department of Rome-DNA Unit, Rome, Italy
Iacovacci, G
Carabinieri Scientific Investigation Department of Rome-DNA Unit, Rome, Italy
Franchi, C
Carabinieri Scientific Investigation Department of Rome-DNA Unit, Rome, Italy
Rapone, C
Carabinieri Scientific Investigation Department of Rome-DNA Unit, Rome, Italy
Di Carlo, A
Carabinieri Scientific Investigation Department of Rome-Fingerprint Unit, Rome, Italy
Oddo, CM
Carabinieri Headquarter-Healt Department, Rome, Italy
Lago, G
Carabinieri Scientific Investigation Department of Rome-DNA Unit, Rome, Italy
Pages: 3
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Stock #: JFS2005237
ISSN: 0022-1198
DOI: 10.1520/JFS2005237