Genetic Analysis of 15 STR Loci of Chinese Uigur Ethnic Population

    Volume 50, Issue 5 (September 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 17 August 2005

    Page Count: 2


    Zhu, B

    Zhu, J

    Eli Sedike, M
    The First Hospital of Xinjiang Medical College, Xinjiang,

    Huang, P

    Wu, Q
    Shaanxi Blood Center, Shaanxi,

    Liu, Y
    Institute of Forensic Science, Beijing,

    Xu, Y

    Wang, Z

    (Received 12 March 2005; accepted 23 April 2005)

    Abstract

    The blood samples were obtained from 106 unrelated healthy individuals from Yili Uigur ethnic autonomous region, Xin Jiang Province of China. Genomic DNA was extracted using the Chelex100 protocol as described by Walsh et al. (1). PCR for 15 STRs was performed in multiplex reaction using AmpFLSTR Identifiler kit; 0.9 µL (2 ng/µL) genomic DNA samples were amplified in a total reaction volume of 10 µL along with 2.9 µL deionized water, 4 µL dNTP, 0.2 µL AmpliTaqGold DNA polymerase, and 2.0 µL primer set. Thermal cycling was conducted with the below conditions: 95°C for 11 min; 28 cycles of 94°C for 60 sec, 59°C for 60 sec, 72°C for 60 sec; and a final extension of 60°C for 45min. Detection and genotyping of all PCR products were accomplished using ABI3100 DNA Genetic Analyzer (Applied Biosystem). Allele designation was done using GeneScan3.7 and Genotyper3.7.


    Paper ID: JFS2005184

    DOI: 10.1520/JFS2005184

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    Author
    Title Genetic Analysis of 15 STR Loci of Chinese Uigur Ethnic Population
    Symposium , 0000-00-00
    Committee E30