ISSN: 0022-1198
CODEN: JFSCA
Published Online: 17 August 2005
Page Count: 4

STR Data for the PowerPlex® 16 System Loci for the Malays, Chinese and Indians Groups of the Malaysian Population
Seah, LH
Jalan Sultan,

Izuan, M
Jalan Sultan,

Nor, NM
Universiti Sains Malaysia,

Panneerchelvam, S
Universiti Sains Malaysia,

(Received 12 March 2005; accepted 23 April 2005)

Abstract

A STR database for use in forensic casework was created for three major ethnic population groups in Malaysia. Blood samples were collected from unrelated individuals and DNA were extracted using Chelex method (1). PCR amplification was performed following manufactures's instructions (PowerPlex® 16 System, Promega Corporation). The amplified products were analyzed and detected using the ABIPRISM® 3100 Gene Analyzer (Applied Biosystem) with 3100 Data Collection Software (Version 1.1). The results were analyzed with GeneScan analysis software (Version 3.7.1) and genotypes were determined by comparison to allelic ladder (Promega Corporation) using Genotyper DNA fragment analysis software (Version 3.7) with the PowerTyperTM Macros (Promega Corporation).

Keywords:
forensic science, short tandem repeat(STR), population genetics, Malays, Chinese, Indians, population, PowerPlex® 16 system, DNA typing

Paper ID: JFS2005170
DOI: 10.1520/JFS2005170
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