Volume 50, Issue 5 (September 2005)
STR Data for the PowerPlex® 16 System Loci for the Malays, Chinese and Indians Groups of the Malaysian Population
A STR database for use in forensic casework was created for three major ethnic population groups in Malaysia. Blood samples were collected from unrelated individuals and DNA were extracted using Chelex method (1). PCR amplification was performed following manufactures's instructions (PowerPlex® 16 System, Promega Corporation). The amplified products were analyzed and detected using the ABIPRISM® 3100 Gene Analyzer (Applied Biosystem) with 3100 Data Collection Software (Version 1.1). The results were analyzed with GeneScan analysis software (Version 3.7.1) and genotypes were determined by comparison to allelic ladder (Promega Corporation) using Genotyper DNA fragment analysis software (Version 3.7) with the PowerTyperTM Macros (Promega Corporation).