Volume 50, Issue 5 (September 2005)
Autosomal STR Polymorphism in Sikh Population of Peninsular Malaysia
Buccal swabs were collected from random ethnic Sikh individuals. Sikh population is a distinct group in the Malaysian Indian population of Peninsular Malaysia. DNA was extracted by simple salting out procedure (1). Using STR multiplex primer kits, Promega Geneprint™ (CTT, FFv and STR III) and following the manufacturer's guidelines 10 ng of DNA was PCR amplified. Allele frequencies were calculated from the numbers of each genotype by gene count method. The randomness of the population was ascertained by subjecting the data to Chi-square test. No deviations from Hardy-Weinberg equilibrium were observed. Heterozygosity and discrimination power were calculated as per the methods already reported (2–4). The allelic distribution in the Sikh population of Malaysia is compared with the Tamil population, which is an another sub-population group of Malaysian Indian population in peninsular Malaysia (5).