Volume 50, Issue 5 (September 2005)
Rapid Determination of Carbohydrates in Heroin Drug Seizures Using Capillary Electrophoresis with Short-End Injection
A simple and rapid method for the analysis of carbohydrates in heroin samples by capillary electrophoresis utilizing a borate complexation method is described. Separations were performed using an uncoated fused silica capillary, 50 cm × 50 μm I.D. × 360μm O.D. with an effective separation length of 9 cm. The system was run at 60°C with an applied voltage of −8 kilovolts. Injection of each sample was for 1 sec at −50 mbar. UV detection was employed with the wavelength set at 195 nm. The background electrolyte consisted of 65 mM borate, pH 12.0. Samples and standards were prepared in the run buffer containing 2 mg/mL of mannose as an internal standard. Under these conditions a test mixture containing glucose, sucrose, lactose, mannitol and mannose as an internal standard was resolved within 5 min. The method was used to determine the concentration of carbohydrates in heroin seizure samples and synthetic heroin samples. The results were in good agreement with the reported values.