You are being redirected because this document is part of your ASTM Compass® subscription.
    This document is part of your ASTM Compass® subscription.

    Volume 50, Issue 5 (September 2005)

    An Alu-based, MGB Eclipse™ Real-Time PCR Method for Quantitation of Human DNA in Forensic Samples

    (Received 17 December 2004; accepted 23 April 2005)

    Published Online: August

    CODEN: JFSOAD

      Format Pages Price  
    PDF 10 $25   ADD TO CART

    Cite this document

    X Add email address send
    X
      .RIS For RefWorks, EndNote, ProCite, Reference Manager, Zoteo, and many others.   .DOCX For Microsoft Word



    Abstract

    The forensic community needs quick, reliable methods to quantitate human DNA in crime scene samples to replace the laborious and imprecise slot blot method. A real-time PCR based method has the possibility of allowing development of a faster and more quantitative assay. Alu sequences are primate-specific and are found in many copies in the human genome, making these sequences an excellent target or marker for human DNA. This paper describes the development of a real-time Alu sequence-based assay using MGB Eclipse™ primers and probes. The advantages of this assay are simplicity, speed, less hands-on-time and automated quantitation, as well as a large dynamic range (128 ng/µL to 0.5 pg/µL).


    Author Information:

    Buel, E
    Department of Public Safety, Waterbury, VT

    Nicklas, JA
    Department of Public Safety, Waterbury, VT


    Stock #: JFS2004514

    ISSN: 0022-1198

    DOI: 10.1520/JFS2004514

    ASTM International
    is a member of CrossRef.

    Author
    Title An Alu-based, MGB Eclipse™ Real-Time PCR Method for Quantitation of Human DNA in Forensic Samples
    Symposium , 0000-00-00
    Committee E30