An Alu-based, MGB Eclipse™ Real-Time PCR Method for Quantitation of Human DNA in Forensic Samples

    Volume 50, Issue 5 (September 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 17 August 2005

    Page Count: 10


    Buel, E
    Department of Public Safety, Waterbury, VT

    Nicklas, JA
    Department of Public Safety, Waterbury, VT

    (Received 17 December 2004; accepted 23 April 2005)

    Abstract

    The forensic community needs quick, reliable methods to quantitate human DNA in crime scene samples to replace the laborious and imprecise slot blot method. A real-time PCR based method has the possibility of allowing development of a faster and more quantitative assay. Alu sequences are primate-specific and are found in many copies in the human genome, making these sequences an excellent target or marker for human DNA. This paper describes the development of a real-time Alu sequence-based assay using MGB Eclipse™ primers and probes. The advantages of this assay are simplicity, speed, less hands-on-time and automated quantitation, as well as a large dynamic range (128 ng/µL to 0.5 pg/µL).


    Paper ID: JFS2004514

    DOI: 10.1520/JFS2004514

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    Title An Alu-based, MGB Eclipse™ Real-Time PCR Method for Quantitation of Human DNA in Forensic Samples
    Symposium , 0000-00-00
    Committee E30