Volume 50, Issue 3 (May 2005)

    Forensic Casework Analysis Using the HVI/HVII mtDNA Linear Array Assay

    (Received 30 November 2004; accepted 9 January 2005)

    Published Online: April

    CODEN: JFSOAD

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    Abstract

    The mitochondrial hypervariable regions I and II have proven to be a useful target for analysis of forensic materials, in which the amount of DNA is limited or highly degraded. Conventional mitochondrial DNA (mtDNA) sequencing can be time-consuming and expensive, limitations that can be minimized using a faster and less expensive typing assay.We have evaluated the exclusion capacity of the linear array mtDNA HVI/HVII region-sequence typing assay (Roche Applied Science) in 16 forensic cases comprising 90 samples. Using the HVI/HVII mtDNA linear array, 56% of the samples were excluded and thus less than half of the samples require further sequencing due to a match or inconclusive results. Of all the samples that were excluded by sequence analysis, 79% could be excluded using the HVI/HVII linear array alone. Using the HVI/HVII mtDNA linear array assay, we demonstrate the potential to decrease sequencing efforts substantially and thereby reduce the cost and the turn-around time in casework analysis.


    Author Information:

    Divne, A-M
    Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala,

    Calloway, C
    Roche Molecular Systems, Alameda, CA

    Erlich, H
    Roche Molecular Systems, Alameda, CA

    Allen, M
    Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala,

    Nilsson, M
    Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala,

    Reynolds, R
    Roche Molecular Systems, Alameda, CA


    Stock #: JFS2004505

    ISSN: 0022-1198

    DOI: 10.1520/JFS2004505

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    Author
    Title Forensic Casework Analysis Using the HVI/HVII mtDNA Linear Array Assay
    Symposium , 0000-00-00
    Committee E30