Volume 50, Issue 4 (July 2005)

    Successful DNA Typing of Urine Stains Using a DNA Purification Kit Following Dialfiltration

    (Received 14 November 2004; accepted 20 February 2005)

    Published Online: May

    CODEN: JFSOAD

      Format Pages Price  
    PDF Version 5 $25   ADD TO CART


    Abstract

    To evaluate the utility of DNA polymorphism typing of urine stains in forensic investigations, the amplifiable amount of DNA was estimated in 20 urine specimens obtained from 10 male and 10 female volunteers using a DNA purification kit following dialfiltration. DNA obtained from both urine and urine stains was amplified with the AmpflSTR® Profiler™ PCR Amplification Kit, and was analyzed by capillary electrophoresis using the Genetic Analyzer. The amount of male and female urine necessary for obtaining a complete DNA profile was 0.2mL and 0.08 mL, respectively. When 0.2mL of male urine were used to create urine stains, complete DNA profiles could be obtained from just some of the stains. However, when only 0.1mL of female urine was used, complete profiles could be successfully obtained from all of the stains. DNA on bleached cotton remained amplifiable for 3–6 weeks. This method using a DNA purification kit following dialfiltration can be recommended for the genotyping of urine stains.


    Author Information:

    Miyoshi, A
    Fukuoka University School of Medicine, Jonan-ku, Fukuoka,

    Hara, K
    Fukuoka University School of Medicine, Jonan-ku, Fukuoka,

    Kashimura, S
    Fukuoka University School of Medicine, Jonan-ku, Fukuoka,

    Nakazono, T

    Hayashiba, Y
    Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, Hakata-ku, Fukuoka,


    Stock #: JFS2004484

    ISSN: 0022-1198

    DOI: 10.1520/JFS2004484

    ASTM International
    is a member of CrossRef.

    Author
    Title Successful DNA Typing of Urine Stains Using a DNA Purification Kit Following Dialfiltration
    Symposium , 0000-00-00
    Committee E30