Successful DNA Typing of Urine Stains Using a DNA Purification Kit Following Dialfiltration

    Volume 50, Issue 4 (July 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 25 May 2005

    Page Count: 5


    Miyoshi, A
    Fukuoka University School of Medicine, Jonan-ku, Fukuoka,

    Hara, K
    Fukuoka University School of Medicine, Jonan-ku, Fukuoka,

    Kashimura, S
    Fukuoka University School of Medicine, Jonan-ku, Fukuoka,

    Nakazono, T

    Hayashiba, Y
    Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, Hakata-ku, Fukuoka,

    (Received 14 November 2004; accepted 20 February 2005)

    Abstract

    To evaluate the utility of DNA polymorphism typing of urine stains in forensic investigations, the amplifiable amount of DNA was estimated in 20 urine specimens obtained from 10 male and 10 female volunteers using a DNA purification kit following dialfiltration. DNA obtained from both urine and urine stains was amplified with the AmpflSTR® Profiler™ PCR Amplification Kit, and was analyzed by capillary electrophoresis using the Genetic Analyzer. The amount of male and female urine necessary for obtaining a complete DNA profile was 0.2mL and 0.08 mL, respectively. When 0.2mL of male urine were used to create urine stains, complete DNA profiles could be obtained from just some of the stains. However, when only 0.1mL of female urine was used, complete profiles could be successfully obtained from all of the stains. DNA on bleached cotton remained amplifiable for 3–6 weeks. This method using a DNA purification kit following dialfiltration can be recommended for the genotyping of urine stains.


    Paper ID: JFS2004484

    DOI: 10.1520/JFS2004484

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    Title Successful DNA Typing of Urine Stains Using a DNA Purification Kit Following Dialfiltration
    Symposium , 0000-00-00
    Committee E30