Journal Published Online: 25 May 2005
Volume 50, Issue 4

Successful DNA Typing of Urine Stains Using a DNA Purification Kit Following Dialfiltration

CODEN: JFSCAS

Abstract

To evaluate the utility of DNA polymorphism typing of urine stains in forensic investigations, the amplifiable amount of DNA was estimated in 20 urine specimens obtained from 10 male and 10 female volunteers using a DNA purification kit following dialfiltration. DNA obtained from both urine and urine stains was amplified with the AmpflSTR® Profiler™ PCR Amplification Kit, and was analyzed by capillary electrophoresis using the Genetic Analyzer. The amount of male and female urine necessary for obtaining a complete DNA profile was 0.2mL and 0.08 mL, respectively. When 0.2mL of male urine were used to create urine stains, complete DNA profiles could be obtained from just some of the stains. However, when only 0.1mL of female urine was used, complete profiles could be successfully obtained from all of the stains. DNA on bleached cotton remained amplifiable for 3–6 weeks. This method using a DNA purification kit following dialfiltration can be recommended for the genotyping of urine stains.

Author Information

Nakazono, T
Fukuoka University School of Medicine, Jonan-ku, Fukuoka, Japan Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, Hakata-ku, Fukuoka, Japan
Kashimura, S
Fukuoka University School of Medicine, Jonan-ku, Fukuoka, Japan
Hayashiba, Y
Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, Hakata-ku, Fukuoka, Japan
Hara, K
Fukuoka University School of Medicine, Jonan-ku, Fukuoka, Japan
Miyoshi, A
Fukuoka University School of Medicine, Jonan-ku, Fukuoka, Japan
Pages: 5
Price: $25.00
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Stock #: JFS2004484
ISSN: 0022-1198
DOI: 10.1520/JFS2004484