ISSN: 0022-1198
CODEN: JFSCA
Published Online: 25 May 2005
Page Count: 17

Developmental Validation of the Quantifiler™ Real-Time PCR Kits for the Quantification of Human Nuclear DNA Samples
Boland, C
Applied Biosystems, CA

Roinestad, IC
Applied Biosystems, CA

Hennessy, LK
Applied Biosystems, CA

Green, RL
Applied Biosystems, CA

(Received 13 October 2004; accepted 5 February 2005)

Abstract

The Quantifiler™ Human DNA Quantification Kit and the Quantifiler™ Y Human Male DNA Quantification Kit were designed for the quantification of human genomic DNA in forensic samples. The kits use a real-time PCR-based process to quantify, respectively, total human DNA or human male DNA only.We report the results of a developmental validation study that we performed with the Quantifiler Kits, following the official SWGDAM guidelines. The Quantifiler Kits were tested for performance criteria such as species specificity, sensitivity, stability, precision and accuracy, and in addition, were tested with forensic case-type samples and mixed (male:female) DNA samples. The Quantifiler Kit methods were highly specific for human DNA, and could detect as little as 32 picograms of DNA using 2 μL of sample per assay. The accuracy and precision of the Quantifiler Kit methods was comparable or superior to that of other quantification methods.

Keywords:
forensic science, DNA quantification, forensic DNA analysis, polymerase chain reaction

Paper ID: JFS2004478
DOI: 10.1520/JFS2004478
ASTM International is a member of CrossRef.