Developmental Validation of the Quantifiler™ Real-Time PCR Kits for the Quantification of Human Nuclear DNA Samples

    Volume 50, Issue 4 (July 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 25 May 2005

    Page Count: 17


    Boland, C
    Applied Biosystems, Foster City, CA

    Roinestad, IC
    Applied Biosystems, Foster City, CA

    Hennessy, LK
    Applied Biosystems, Foster City, CA

    Green, RL
    Applied Biosystems, Foster City, CA

    (Received 13 October 2004; accepted 5 February 2005)

    Abstract

    The Quantifiler™ Human DNA Quantification Kit and the Quantifiler™ Y Human Male DNA Quantification Kit were designed for the quantification of human genomic DNA in forensic samples. The kits use a real-time PCR-based process to quantify, respectively, total human DNA or human male DNA only.We report the results of a developmental validation study that we performed with the Quantifiler Kits, following the official SWGDAM guidelines. The Quantifiler Kits were tested for performance criteria such as species specificity, sensitivity, stability, precision and accuracy, and in addition, were tested with forensic case-type samples and mixed (male:female) DNA samples. The Quantifiler Kit methods were highly specific for human DNA, and could detect as little as 32 picograms of DNA using 2 μL of sample per assay. The accuracy and precision of the Quantifiler Kit methods was comparable or superior to that of other quantification methods.


    Paper ID: JFS2004478

    DOI: 10.1520/JFS2004478

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    Title Developmental Validation of the Quantifiler™ Real-Time PCR Kits for the Quantification of Human Nuclear DNA Samples
    Symposium , 0000-00-00
    Committee E30