ISSN: 0022-1198
CODEN: JFSCA
Published Online: 25 May 2005
Page Count: 7

STR-Typing of Human DNA from Human Fecal Matter Using the QIAGEN QIAamp® Stool Mini Kit
Johnson, DJ

Roberts, KA

Martin, RL
School of Criminal Justice and Criminalistics, California State University, Los Angeles, College of Health and Human Services, CA

(Received 2 October 2004; accepted 22 January 2005)

Abstract

The purpose of this study was to compare the effectiveness of the QIAGEN QIAamp® Stool Mini Kit against a standard phenolchloroform procedure for the extraction, quantitation, and STR-typing of human nuclear DNA from human feces. Stools from six subjects were sampled by swabbing and excision. Samples extracted with the QIAamp kit gave a wide range of DNA yields, whereas those extracted by the organic method yielded no DNA. DNA was not recovered from one subject's stools by either procedure. The QIAamp extracts were amplified with the Profiler Plus™ and COfiler™ kits, and PCR inhibition was observed with DNA extracts that were further concentrated. Substitution of water or TE-4 for the QIAamp elution buffer eliminated most, if not all, of the inhibition. A modified QIAamp procedure was used to extract thirty samples, which were subjected to one of five environmental conditions. DNA was recovered from all of these samples, and typing results were obtained on 93% of the samples.

Keywords:
forensic science, short tandem repeat typing, fecal matter, polymerase chain reaction inhibition, environmental conditions

Paper ID: JFS2004428
DOI: 10.1520/JFS2004428
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