Strategy for the Estimation of Chronological Age Using the Aspartic Acid Racemization Method with Special Reference to Coefficient of Correlation Between D/L Ratios and Ages

    Volume 50, Issue 5 (September 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 17 August 2005

    Page Count: 8


    Ohtani, S
    Kanagawa Dental College, Kanagawa,

    Yamamoto, T
    Kanagawa Dental College, Kanagawa,

    (Received 2 September 2004; accepted 9 April 2005)

    Abstract

    The estimation of chronological age has been performed by various methods in forensic science. Among these, racemization methods, which are based on the age-dependent non-enzymatic changes of L-form amino acids to D-form mainly using aspartic acid, are one of the most reliable and accurate methods to date. Separation of enantiomers is generally performed by gas chromatography or high performance liquid chromatography. Various tissues with low metabolic rates have been applied for this purpose. In addition, single proteins purified from these target tissues are also applicable. In this brief review we describe this method in detail, noting points of caution, as well as the advantages and disadvantages of the different target tissues. In addition, special attention is given to the correlation rates obtained between chronological age and enantiomer ratios. Currently, based on accuracy of estimated age, simplicity of the method, time required, and reproducibility, tooth dentin is considered one of the best target tissues. Alternatively, analysis of osteocalcin and elastin have also provided accurate and reproducible results.


    Paper ID: JFS2004354

    DOI: 10.1520/JFS2004354

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    Title Strategy for the Estimation of Chronological Age Using the Aspartic Acid Racemization Method with Special Reference to Coefficient of Correlation Between D/L Ratios and Ages
    Symposium , 0000-00-00
    Committee E30