Liquid Chromatography-Mass Spectrometric and Liquid Chromatography-Tandem Mass Spectrometric Determination of Hallucinogenic Indoles Psilocin and Psilocybin in “Magic Mushroom” Samples

    Volume 50, Issue 2 (January 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 5 January 2005

    Page Count: 5


    Tsuchihashi, H
    Forensic Science Laboratory, Osaka Prefectural Police H.Q., Osaka,

    Katagi, M
    Forensic Science Laboratory, Osaka Prefectural Police H.Q., Osaka,

    Nishikawa, M
    Forensic Science Laboratory, Osaka Prefectural Police H.Q., Osaka,

    Kamata, T
    Forensic Science Laboratory, Osaka Prefectural Police H.Q., Osaka,

    (Received 3 July 2004; accepted 2 October 2004)

    Abstract

    Accurate and sensitive analytical methods for psilocin (PC) and psilocybin (PB), tryptamine-type hallucinogens contained in “magic mushrooms,” were investigated using liquid chromatography-mass spectrometry (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS-MS). The chromatographic separation on an ODS column and mass spectral information gave complete discrimination between PC and PB without derivatization. The mass spectrometric detection had a high sensitivity, and the tandem mass spectrometric detection provided more specificity and accuracy, as well as high sensitivity. The detection limits ranged from 1 to 25 pg by LC-MS in the selected ion monitoring mode, and the intra- and inter-day coefficients of variation were estimated to be 4.21–5.93% by LC-MS-MS in the selected reaction monitoring mode. By applying the present LC-MS-MS technique to four real samples, the contents of PC and PB were found to vary over a wide range (0.60–1.4 and 0.18–3.8 mg/g dry wt. for PC and PB, respectively) between samples.


    Paper ID: JFS2004276

    DOI: 10.1520/JFS2004276

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    Title Liquid Chromatography-Mass Spectrometric and Liquid Chromatography-Tandem Mass Spectrometric Determination of Hallucinogenic Indoles Psilocin and Psilocybin in “Magic Mushroom” Samples
    Symposium , 0000-00-00
    Committee E30