Simultaneous Separation of Different Types of Amphetamine and Piperazine Designer Drugs by Capillary Electrophoresis with a Chiral Selector

    Volume 50, Issue 2 (March 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 March 2005

    Page Count: 10


    McCord, BR
    International Forensic Research Institute, Miami, FL

    Loeliger, JR
    U.S. Food and Drug Administration, Forensic Chemistry Center, Cincinnati, OH

    Witkowski, MR
    U.S. Food and Drug Administration, Forensic Chemistry Center, Cincinnati, OH

    Bishop, SC
    Ohio University, Athens, OH

    Gratz, SR
    U.S. Food and Drug Administration, Forensic Chemistry Center, Cincinnati, OH

    (Received 13 June 2004; accepted 25 September 2004)

    Abstract

    The recent emergence of a new class of piperazine-type compounds has brought about the need for laboratory screening methods for both seized drugs and toxicological samples. These piperazine compounds, which include 1-benzylpiperazine (BZP) and 1-(3-trifluoromethylphenyl) piperazine (TFMPP), exhibit comparable physiological effects and can be substituted for the classic amphetamine-type drugs. We have optimized a chiral capillary electrophoresis (CE) separation that detects a set of 6 piperazine and 4 chiral amphetamine compounds in under 23 min using a 200 mM phosphate buffer at a pH = 2.8 with 20 mM hydroxypropyl-β-cyclodextrin (HPβCD). In addition to the above compounds, a series of “clandestine” BZP diHCl samples were also analyzed using this method to assess the ruggedness of the procedure. The novel CE separation was tailored to simultaneously detect these piperzine compounds in addition to amphetamine-type drugs. Distinct migration time and UV-spectral data were obtained for all compounds of interest.


    Paper ID: JFS2004239

    DOI: 10.1520/JFS2004239

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    Title Simultaneous Separation of Different Types of Amphetamine and Piperazine Designer Drugs by Capillary Electrophoresis with a Chiral Selector
    Symposium , 0000-00-00
    Committee E30