1H NMR Analysis of GHB and GBL: Further Findings on the Interconversion and a Preliminary Report on the Analysis of GHB in Serum and Urine

    Volume 50, Issue 1 (January 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 2005

    Page Count: 6


    Del Signore, AG
    College of Pharmacy, University of Rhode Island, Kingston, RI

    Cho, BP
    College of Pharmacy, University of Rhode Island, Kingston, RI

    McGregor, M
    University of Rhode Island, Kingston, RI

    (Received 8 May 2004; accepted 14 August 2004)

    Abstract

    A 1H nuclear magnetic resonance (1H NMR) method for the determination of gamma-hydroxybutyric acid (GHB) and gamma-hydroxybutyrolactone (GBL) in human serum and urine using spiked samples has been developed. The method gives linear responses (correlation coefficients of 0.99 or greater) over the concentration range 0.01 mg/mL to 4.0 mg/mL in urine and 0.3 mg/mL to 2.0 mg/mL in serum. No sample pretreatment is required. Studies of the chemical interconversion of GBL and GHB showed hydrolysis of GBL to be rapid at pH 11.54, slower and less complete (30% hydrolysis) at pH 2.54 and slowest at pH 7.0, reaching 30% hydrolysis in about 40 days. No esterification of GHB was observed at any pH.


    Paper ID: JFS2004179

    DOI: 10.1520/JFS2004179

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    Title 1H NMR Analysis of GHB and GBL: Further Findings on the Interconversion and a Preliminary Report on the Analysis of GHB in Serum and Urine
    Symposium , 0000-00-00
    Committee E30