Volume 50, Issue 3 (May 2005)

    Multiplex PCR Development of Y-chromosomal Biallelic Polymorphisms for Forensic Application

    (Received 3 April 2004; accepted 16 October 2004)

    Published Online: April

    CODEN: JFSOAD

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    Abstract

    Single-nucleotide polymorphisms of Y chromosome (Y-SNPs) are a class of markers of interest in forensic investigations, because many of them show regional specificity, providing useful information about the geographic origin of a subject or evidence under investigation. A first multiplex with 7 SNPs (M35, M89, M9, M170, M172, M45, M173), which occur in the basal branches of the phylogenetic tree and are able to assign a subject to known most frequent European haplogroups, was designed. SNP genotyping was accomplished by hot-start PCR with primers amplifying fragments between 96 and 136 nucleotides, minisequencing, and capillary electrophoresis of extension products. Ninety seven subjects of known geographic provenance were studied, of which 68 from Europe. Of these, 57 had mutations found more frequently in European haplogroups and 11 more frequent in Asian populations. Subjects from non-European countries were also examined and had haplogroups common in their regions of provenance. Experiments with low molecular weight DNA gave positive amplification from 1 ng of DNA for all seven SNPs.


    Author Information:

    Tagliabracci, A
    Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona,

    Turchi, C
    Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona,

    Alessandrini, F
    Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona,

    Buscemi, L
    Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona,

    Pesaresi, M
    Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona,

    Onofri, V
    Istituto di Medicina Legale, Università Politecnica delle Marche, Ancona,


    Stock #: JFS2004147

    ISSN: 0022-1198

    DOI: 10.1520/JFS2004147

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    Title Multiplex PCR Development of Y-chromosomal Biallelic Polymorphisms for Forensic Application
    Symposium , 0000-00-00
    Committee E30