Volume 49, Issue 4 (July 2004)

    A Minimally Destructive Technique for Sampling Dentin Powder for Mitochondrial DNA Testing

    (Received 30 December 2003; accepted 1 January 2004)

    Published Online: May

    CODEN: JFSOAD

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    Abstract

    The purpose of this paper is to present the horizontal sectioning technique used by odontologists at the Central Identification Laboratory to sample dentin for mtDNA analysis. From the perspective of DNA testing, anthropologists and odontologists at the Central Identification Laboratory work with ancient remains. In many instances, the lack of comprehensive antemortem records, the potential for fragmentation and commingling, and environmental exposure makes the use of traditional forensic identification techniques difficult or impossible. Teeth are highly resistant to environmental degradation and are an excellent source of mitochondrial DNA (mtDNA). This technique is simple, quick, and relatively conservative, allowing for preservation of the majority of the external portion of the tooth structure.


    Author Information:

    Fielding, CG
    Chief, Oral and Maxillofacial Pathology, Fort Gordon, Georgia

    Shiroma, CY
    Formerly forensic odontologist, Joint POW/MIA Accounting Command, Hickam Air Force Base, Hawaii

    Lewis, JA
    Forensic Odontologist, Hickam Air Force Base, Hawaii

    Dunn, KN
    Forensic Odontologist, Hickam Air Force Base, Hawaii

    Gleisner, MR
    Forensic Odontologist, Hickam Air Force Base, Hawaii


    Stock #: JFS2003432

    ISSN: 0022-1198

    DOI: 10.1520/JFS2003432

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    Title A Minimally Destructive Technique for Sampling Dentin Powder for Mitochondrial DNA Testing
    Symposium , 0000-00-00
    Committee E30