ISSN: 0022-1198
CODEN: JFSCA
Published Online: 5 May 2004
Page Count: 5

A Minimally Destructive Technique for Sampling Dentin Powder for Mitochondrial DNA Testing
Fielding, CG
Chief, Oral and Maxillofacial Pathology, Georgia

Shiroma, CY
Formerly forensic odontologist, Joint POW/MIA Accounting Command, Hawaii

Lewis, JA
Forensic Odontologist, Hawaii

Dunn, KN
Forensic Odontologist, Hawaii

Gleisner, MR
Forensic Odontologist, Hawaii

(Received 30 December 2003; accepted 1 January 2004)

Abstract

The purpose of this paper is to present the horizontal sectioning technique used by odontologists at the Central Identification Laboratory to sample dentin for mtDNA analysis. From the perspective of DNA testing, anthropologists and odontologists at the Central Identification Laboratory work with ancient remains. In many instances, the lack of comprehensive antemortem records, the potential for fragmentation and commingling, and environmental exposure makes the use of traditional forensic identification techniques difficult or impossible. Teeth are highly resistant to environmental degradation and are an excellent source of mitochondrial DNA (mtDNA). This technique is simple, quick, and relatively conservative, allowing for preservation of the majority of the external portion of the tooth structure.

Keywords:
forensic science, forensic odontology, mitochondrial DNA, dentin sampling, JPAC, Central Identification Laboratory

Paper ID: JFS2003432
DOI: 10.1520/JFS2003432
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