Comparison of Age Estimated from Degree of Racemization of Aspartic Acid, Glutamic Acid and Alanine in the Femur

    Volume 49, Issue 3 (May 2004)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 10 March 2004

    Page Count: 5


    Gonmori, K
    Akita University School of Medicine, Akita City, Akita

    Yoshioka, N
    Akita University School of Medicine, Akita City, Akita

    Arany, S
    Akita University School of Medicine, Akita City, Akita

    Ohtani, S
    Kanagawa Dental College, Yokosuka, Kanagawa

    Yamamoto, T
    Kanagawa Dental College, Yokosuka, Kanagawa

    Yamada, Y
    Kanagawa Dental College, Yokosuka, Kanagawa

    (Received 14 December 2003; accepted 16 January 2003)

    Abstract

    Aspartic acid (Asp) is generally used for estimation of age by measuring the degree of racemization. For other amino acids, however, there are few reports regarding the usefulness of the degree of racemization for the estimation of age. Accordingly, in this study using the femur (obtained from 21 cadavers) as the specimen, we measured the degree of racemization of glutamic acid (Glu) and alanine (Ala) along with Asp in the total amino acid (TAA) fraction as well as in acid-insoluble collagen-rich (IC) and acid-soluble peptide (SP) sub-fractions. We compared the degrees of racemization of each amino acid and the accuracy of the ages estimated from them. The degree of racemization and the reaction rate of racemization were ranked in the order of Asp>Glu>Ala in the TAA and IC fractions, but Asp>Ala>Glu in the SP fraction. It is noteworthy that the degrees of racemization differed between the three amino acids depending on the fraction tested. The correlation coefficient (r) between the degree of racemization and the chronological age was higher in the SP than in the TAA or IC fraction. Among three amino acids, Asp showed the highest correlation coefficient as predicted. The present study confirmed that Asp from the SP fraction is the best indicator for age estimation using racemization rates.


    Paper ID: JFS2003414

    DOI: 10.1520/JFS2003414

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    Title Comparison of Age Estimated from Degree of Racemization of Aspartic Acid, Glutamic Acid and Alanine in the Femur
    Symposium , 0000-00-00
    Committee E30