Use of Solid-Phase Double-Antibody Radioimmunoassay to Identify Species from Small Skeletal Fragments

    Volume 49, Issue 5 (September 2004)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 September 2004

    Page Count: 6


    Hood, DG
    MicroAnalytica, LLC, Miami, FL

    Ubelaker, DH
    Smithsonian Institution, NMNH, Washington, D.C.

    Lowenstein, JM
    California Pacific Medical Center, San Francisco, CA

    (Received 10 December 2003; accepted 7 May 2004)

    Abstract

    Protein radioimmunoassay (pRIA) offers the potential to identify species in small skeletal fragments submitted as forensic evidence. The technique consists of protein extraction followed by a solid-phase double-antibody radioimmunoassay using controls of antisera (raised in rabbits) and radioactive (iodine-125) antibody of rabbit gamma globulin (produced in donkeys). Species determination results from evaluation of radioactivity uptake. To demonstrate the potential of this technique, six known bone samples (three human and three nonhuman, including one from a deer [Odocoileus virginianus]) were submitted for blind analysis. pRIA correctly distinguished the human from the nonhuman samples. Using 200 mg or less of each sample, species of the deer specimen was identified correctly, given the choices of cow, deer, dog, goat, and pig.


    Paper ID: JFS2003399

    DOI: 10.1520/JFS2003399

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    Title Use of Solid-Phase Double-Antibody Radioimmunoassay to Identify Species from Small Skeletal Fragments
    Symposium , 0000-00-00
    Committee E30