Pancuronium Bromide (Pavulon) Isolation and Identification in Aged Autopsy Tissues and Fluids

    Volume 50, Issue 1 (January 2005)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 2005

    Page Count: 8


    Alcaraz, A
    Forensic Science Center, Livermore National Laboratory, Livermore, CA.

    Andresen, BD
    Forensic Science Center, Livermore National Laboratory, Livermore, CA.

    Grant, PM
    Forensic Science Center, Livermore National Laboratory, Livermore, CA.

    (Received 25 January 2003; accepted 31 July 2004)

    Abstract

    The isolation and detection of pancuronium bromide was developed for aged autopsy samples to identify and confirm this compound in questioned tissue samples. A novel protocol was optimized for the isolation of the target drug in highly decomposed tissues. Solid-phase extraction (SPE) cartridges containing styrene-divinylbenzene were investigated. This polymer retained quaternary drugs and facilitated sequential elution upon washing with commonly available solvents. The semi-purified SPE samples were prescreened by pyrolysis GC-MS. A candidate specimen was then confirmed by microbore high-performance liquid chromatography/electrospray-ionization/mass spectrometry (µHPLC-ESI-MS/MS) with a triple-quadrupole mass spectrometer. The developed procedures provided a qualitative or semiquantitative (at best) basis for the investigation of difficult cases involving overdoses of polar drugs.


    Paper ID: JFS2003352

    DOI: 10.1520/JFS2003352

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    Author
    Title Pancuronium Bromide (Pavulon) Isolation and Identification in Aged Autopsy Tissues and Fluids
    Symposium , 0000-00-00
    Committee E30