Validation Study of the True Allele® Automated Data Review System

    Volume 49, Issue 4 (July 2004)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 5 May 2004

    Page Count: 8


    Kozlowski, BE
    Forensic Scientist, New York State Convicted Offender DNA Databank, New York State Police Forensic Investigation Center, Albany, NY

    Duceman, BW
    Director of Biological Science, New York State Police Forensic Investigation Center, Albany, NY

    Kadash, K
    Forensic Scientist, New York State Convicted Offender DNA Databank, New York State Police Forensic Investigation Center, Albany, NY

    Biega, LA
    Supervisor of DNA Services, New York State Convicted Offender DNA Databank, New York State Police Forensic Investigation Center, Albany, NY

    (Received 1 October 2003; accepted 1 February 2004)

    Abstract

    The New York State Convicted Offender DNA Databank is the first U.S. lab to complete an internal validation of the True Allele® expert data review system. TrueAllele® is designed to assess short tandem repeat (STR) DNA data based on several key features such as peak height, shape, area, and position relative to a standard ladder and use this information to make accurate allele calls. The software then prioritizes the allele calls based on several user-defined rules. As a result, the user need only review low-quality data. The validation of this system consisted of an extensive optimization phase and a large concordance phase. During optimization, the rule settings were tailored to minimize the amount of high-quality data viewed by the user. In the concordance phase, a large dataset was typed in parallel with the ABI software Gene Scan® and Genotyper® (manual review) and TrueAllele® (automated review) for comparison of allele calls and sample state assignment. Only one significant difference was discovered out of 2048 samples in the concordance study. In this case, TrueAllele® revealed a spike in the profile that was interpreted as a DNA peak by the analyst in Genotyper®. TrueAllele® was designed to focus the review on poor data and to eliminate the need for complete reanalysis technical review. This validation project proved TrueAllele® to be dependable for use at the NYS Convicted Offender DNA Databank.


    Paper ID: JFS2003336

    DOI: 10.1520/JFS2003336

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    Author
    Title Validation Study of the True Allele® Automated Data Review System
    Symposium , 0000-00-00
    Committee E30