Volume 49, Issue 4 (July 2004)

    Y-SNP Typing of U.S. African American and Caucasian Samples Using Allele-Specific Hybridization and Primer Extension

    (Received 6 September 2003; accepted 6 March 2004)

    Published Online: May

    CODEN: JFSOAD

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    Abstract

    Multiplex analysis of genetic markers has become increasingly important in a number of fields, including DNA diagnostics and human identity testing. Two methods for examination of single nucleotide polymorphisms (SNPs) with a potential for a high degree of multiplex analysis of markers are primer extension with fluorescence detection, and allele-specific hybridization using flow cytometry. In this paper, we examined 50 different SNPs on the Y-chromosome using three primer extension multiplexes and five hybridization multiplex assays. For certain loci, the allele-specific hybridization method exhibited sizable background signal from the absent alternate allele. However, 100% concordance (>2000 alleles) was observed in ten markers that were typed using both methods. A total of 18 unique haplogroups out of a possible 45 were observed in a group of 229 U.S. African American and Caucasian males with the majority of samples being assigned into 2 of the 18 haplogroups.


    Author Information:

    Butler, JM
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD

    Vallone, PM
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD


    Stock #: JFS2003303

    ISSN: 0022-1198

    DOI: 10.1520/JFS2003303

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    Author
    Title Y-SNP Typing of U.S. African American and Caucasian Samples Using Allele-Specific Hybridization and Primer Extension
    Symposium , 0000-00-00
    Committee E30