Volume 49, Issue 4 (July 2004)

    A Simple and Efficient Method for Extracting DNA From Old and Burned Bone

    (Received 16 August 2003; accepted 13 March 2004)

    Published Online: May

    CODEN: JFSOAD

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    Abstract

    It has been a challenge to extract DNA from bones previously soaked in water, burned, or buried for a long time, due to the reduced quality and quantity of DNA in the bone samples. The dramatic degradation of the DNA and the presence of PCR inhibitors in the collagen significantly complicate the process of DNA identification in dated and charred bones. In this article, we present a novel strategy to obtain DNA from bones based on the use of cetyltrimethylammonium bromide (CTAB) lysis buffer and isoamyl alcohol-chloroform extraction with subsequent DNA purification using the DNA IQ™ System, or alternatively the QIAquick™ system. When applied to bones soaked, burned or buried for up to nine years, this method increases the purity and yield of DNA with respect to the traditional phenol-chloroform method and significantly improves multiplex STR genotyping using fluorescence-based methods. The results of this research will assist forensic scientists in the identification of DNA from victims whose bodies underwent significant trauma or burning, prec luding the utilization of traditional forensic DNA identification techniques.


    Author Information:

    Ji, A
    Institute of Forensic Science, Ministry of Public Security, Beijing,

    Jiang, C
    Institute of Forensic Science, Ministry of Public Security, Beijing,

    Parra, EJ
    University of Toronto at Mississauga, Mississauga, ON

    Ye, J
    Institute of Forensic Science, Ministry of Public Security, Beijing,

    Hu, L
    Institute of Forensic Science, Ministry of Public Security, Beijing,

    Zhao, X
    Institute of Forensic Science, Ministry of Public Security, Beijing,

    Zheng, X
    Institute of Forensic Science, Ministry of Public Security, Beijing,

    Tu, Z
    Institute of Forensic Science, Ministry of Public Security, Beijing,


    Stock #: JFS2003275

    ISSN: 0022-1198

    DOI: 10.1520/JFS2003275

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    Author
    Title A Simple and Efficient Method for Extracting DNA From Old and Burned Bone
    Symposium , 0000-00-00
    Committee E30