ISSN: 0022-1198
CODEN: JFSCA
Published Online: 5 May 2004
Page Count: 10

Utility of the Y-STR Typing Systems Y-PLEX ™ 6 and Y-PLEX ™ 5 in Forensic Casework and 11 Y-STR Haplotype Database for Three Major Population Groups in the United States
Lal, A
ReliaGene Technologies, Inc., LA

Paunovic, A
ReliaGene Technologies, Inc., LA

Budowle, B
FBI Laboratory, VA

Larsen, C
ReliaGene Technologies, Inc., LA

Schneida, E
ReliaGene Technologies, Inc., LA

Pineda, G
ReliaGene Technologies, Inc., LA

Nasir, H
ReliaGene Technologies, Inc., LA

Shewale, JG
ReliaGene Technologies, Inc., LA

Shaffer, M
ReliaGene Technologies, Inc., LA

Guidry, RDV
ReliaGene Technologies, Inc., LA

Chakraborty, R
Center for Genome Information,

Sinha, SK
ReliaGene Technologies, Inc., LA

(Received 4 August 2003; accepted 5 February 2004)

Abstract

The Y-PLEX™ 6 and Y-PLEX™ 5 systems enable analysis for 11 Y-STR loci. We present here the utility of these systems in forensic casework. A total of 188 samples, including 127 evidence samples, were analyzed using either or both of the systems. The evidence sample types included fingernail scrapings, sperm or seminal fluid, epithelial cells, blood and other tissues. The Y-STR typing systems provided useful probative results in difficult cases. A reference database for Caucasian (n = 517), African American (n = 535), and Hispanic (n = 245) population groups within the United States was generated for estimating the haplotype frequency in forensic casework. Among the individuals profiled, 311 Caucasians, 412 African Americans, and 194 Hispanics provided unique profiles in their respective population datasets. This is the first report describing the haplotype database for the set of 11 Y-STR loci recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Linkage analysis reveals that the frequencies from forensically important autosomal loci can be multiplied with the Y-STR haplotype frequency. The results from Y-PLEX™systems have been accepted in courts in the United States.

Keywords:
forensic science, Y-chromosome, short tandem repeats, DNA typing, human identification, Y-STR, Y-PLEX, DYS393, DYS19, DYS390, DYS391, DYS385, DYS389I, DYS389II, DYS439, DYS438, DYS392, D2S1388, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, Amelogenin, CSF1PO, DQA1, FGA, GC, GYPA, HBGG, LDLR, Penta D, Penta E, TH01, TPOX, vWA

Paper ID: JFS2003246
DOI: 10.1520/JFS2003246
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