Volume 49, Issue 5 (September 2004)

    Stable Isotope Ratios as a Tool in Microbial Forensics—Part 1. Microbial Isotopic Composition as a Function of Growth Medium?

    (Received 29 June 2003; accepted 28 March 2004)

    Published Online: September

    CODEN: JFSOAD

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    Abstract

    The stable isotope ratios of a seized pathogen culture could potentially reveal information about the environment in which the agent was produced. In this paper we describe general relationships between stable isotopes of carbon, nitrogen, and hydrogen in bacteriological culture media and spores of Bacillus subtilis, an endospore-forming soil bacterium. In numerous media that varied both in nutrient composition and water stable isotope ratios, medium to spore enrichment in carbon isotopes was 0.3 ± 2.0% (parts per thousand), and in nitrogen, 4.5 ± 0.7%. We achieved mass balance for the contribution of hydrogen isotopes from nutrients (70%) and water (30%) to spores in independent experiments by varying the isotope ratios of nutrients or water. A model was derived for predicting the isotope ratio values of spores from those in nutrients and water.


    Author Information:

    Kreuzer-Martin, HW
    Stable Isotope Ratio Facility for Environmental Research, Salt Lake City, Utah

    Ehleringer, JR

    Dorigan, JV
    Central Intelligene Agency, Washington, DC

    Chesson, LA
    Stable Isotope Ratio Facility for Environmental Research, Salt Lake City, Utah

    Lott, MJ


    Stock #: JFS2003226

    ISSN: 0022-1198

    DOI: 10.1520/JFS2003226

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    Title Stable Isotope Ratios as a Tool in Microbial Forensics—Part 1. Microbial Isotopic Composition as a Function of Growth Medium?
    Symposium , 0000-00-00
    Committee E30