Volume 48, Issue 6 (November 2003)
Allele Diversities and Haplotypes of Two Novel Y-STR in a Chinese Population
A total 107 EDTA-blood samples was collected from unrelated males of Han population in Chengdu of China. DNA was extracted utilizing the Chelex-100 method (1). The allelic variation at the two Y-STR loci named as DYS544 and DYS587 were analyzed by PCR amplification. Each PCR reaction contained 2-5 ng human genome, 1 × Taq buffer, 1.5 mM MgCl2, 200 μM each dNTP (Pharmacia Biotech, Sweden), 2 U Taq polymerase (Promega Corporation), 0.3 μM each primer, in a total volume of 37.5 μL. PCR amplifications were carried out in a GeneAmp PCR System 9600 (Perkin-Elmer) with pre-denaturing for 2 min at 94°C, followed by 33 cycles of denaturing for 30 s at 94°C, annealing for 60 s at 58°C, and extension for 30 s at 72°C.