Volume 48, Issue 5 (September 2003)
Gene Frequencies for Two Y-Chromosomal STR Loci in Chinese Population
Blood samples were collected from unrelated males of Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/DYS442 and DYS446.htm. The PCR reaction volume for each locus was 37.5 µL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of population genetics and forensic science were analyzed according to Hou's method (3). The complete dataset can be accessed at http://www.legalmed.org/dna/ DYS442 and DYS446.htm.