Volume 48, Issue 5 (September 2003)
Population Genetics for the CODIS Core STR Loci in the Population of Northeastern Poland
In 1997 the FBI announced the selection of 13 STRs loci to constitute the core of the United States national database-CODIS, which has been widely adopted by forensic DNA analysts in the United States (1). Polish-worldwide population today is estimated at almost 60 million. According to the last U.S. Census taken in year 2000, in the United States of America live about 9,053,660 Poles/Polish-Americans. The largest population of Poles is recorded in the state of New York, Illinois, Michigan, and Pennsylvania (2). The aim of this study was to provide a 13 STRs database for a population sample of Poland for the use as a highly discriminatory system of genetic markers in forensic community. DNA was extracted from the blood specimens of 842 unrelated adult individuals using Chelex-100 and proteinase K (3). Two commercially available kits AmpF1STR Profiler and AmpF1STR SGM Plus (Applera, US) were used to amplify the 13 STR loci included in the CODIS STR standardization project: D3S1358, vWA, D8S1179, D21S11, D18S51, TH01, FGA, TPOX, CSF1P0, D5S818, D13S317, D7S820, D16S539. Genotyping was performed in a 310 ABI Prism Genetic Analyzer. Hardy-Weinberg equilibrium was tested using the Fisher's exact test by the GDA software (4). The forensic efficiency parameters were calculated using the PowerStats software (www.promega.com/techserv/apps).