Published Online: 1 September 2003
Page Count: 2
In 1997 the FBI announced the selection of 13 STRs loci to constitute the core of the United States national database-CODIS, which has been widely adopted by forensic DNA analysts in the United States (1). Polish-worldwide population today is estimated at almost 60 million. According to the last U.S. Census taken in year 2000, in the United States of America live about 9,053,660 Poles/Polish-Americans. The largest population of Poles is recorded in the state of New York, Illinois, Michigan, and Pennsylvania (2). The aim of this study was to provide a 13 STRs database for a population sample of Poland for the use as a highly discriminatory system of genetic markers in forensic community. DNA was extracted from the blood specimens of 842 unrelated adult individuals using Chelex-100 and proteinase K (3). Two commercially available kits AmpF1STR Profiler and AmpF1STR SGM Plus (Applera, US) were used to amplify the 13 STR loci included in the CODIS STR standardization project: D3S1358, vWA, D8S1179, D21S11, D18S51, TH01, FGA, TPOX, CSF1P0, D5S818, D13S317, D7S820, D16S539. Genotyping was performed in a 310 ABI Prism Genetic Analyzer. Hardy-Weinberg equilibrium was tested using the Fisher's exact test by the GDA software (4). The forensic efficiency parameters were calculated using the PowerStats software (www.promega.com/techserv/apps).
Paper ID: JFS2003126