Polymorphism of Two STR Loci on Chromosome 21 in a Chinese Population

    Volume 48, Issue 5 (September 2003)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 September 2003

    Page Count: 1


    Abstract

    Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/d21s1809.htm. The volume of PCR reaction for each locus was 37.5 µL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2,3). Data were analyzed using POWERSTATS program (4). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou's method (5) and no deviation from Hardy-Weinberg equilibrium was observed.


    Paper ID: JFS2003125

    DOI: 10.1520/JFS2003125

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    Author
    Title Polymorphism of Two STR Loci on Chromosome 21 in a Chinese Population
    Symposium , 0000-00-00
    Committee E30